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FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived <t>CD34</t> + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.
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FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived <t>CD34</t> + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.
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FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived <t>CD34</t> + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.
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FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived <t>CD34</t> + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.
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FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived <t>CD34</t> + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.
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FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived <t>CD34</t> + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.
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Image Search Results


FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived CD34 + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.

Journal: Biology Open

Article Title: Site-1 protease ablation in the osterix-lineage in mice results in bone marrow neutrophilia and hematopoietic stem cell alterations

doi: 10.1242/bio.052993

Figure Lengend Snippet: FACS analysis of hematopoietic stem cells in WT and Cko mice. (A) Graph showing the decrease in LIN − cells in the bone marrow of Cko mice when compared to WT. Data are shown as percent of total BMCs analyzed. Graphs showing LSK (B, % of LIN − ), LSK SLAM (C, % of LIN − ), LSK (D, % of total), LSK SLAM (E, % of total), LIN − Sca1 − c-Kit + (F, % of LIN − ), and the LIN − Sca1 − c-Kit + derived CD34 + CD16/32 − (CMP) (G, % of LIN − ) and CD34 + CD16/32 + (GMP) cells (H, % of LIN − ) in the bone marrow of WT and Cko mice. The P -values for the comparison between WT and Cko were calculated using non-parametric t -test (two-tailed) with Mann–Whitney correction from the analyses of WT ( N ≥12) and Cko ( N ≥12) mice from several different litters.

Article Snippet: In addition to FVS575V stain, cells were stained with the following antibodies for 50 min: eFluor 450 conjugated mouse hematopoietic lineage cocktail (Thermo Fisher Scientific, # 88-7772-72), Alexa Fluor 700 conjugated rat anti-mouse Sca1 (Ly6A/E) (Thermo Fisher Scientific, # 56-5981-82), Super Bright 702 conjugated rat anti-mouse c-Kit (clone 2B8) (Thermo Fisher Scientific, # 67-1171-82), PE-Cy7 conjugated rat anti-mouse CD16/32 (Thermo Fisher Scientific, # 25-0161-82), FITC conjugated rat anti-mouse CD34 (Thermo Fisher Scientific, # 11-0341-85), PE conjugated rat anti-mouse CD150 (Thermo Fisher Scientific, # 12-1502-82), and Super Bright 600 conjugated rat anti-mouse CD48 (Thermo Fisher Scientific, # 63-0481-82).

Techniques: Derivative Assay, Comparison, Two Tailed Test, MANN-WHITNEY